In vitro Regeneration of two BINA Tomato (Lycopersicon esculentum Mill.) Varieties of Bangladesh
نویسندگان
چکیده
Cotyledonary leaf from 12 days old aseptically grown seedlings were used as explant. explants of two BINA varieties tomato cultured on MS medium containing different concentrations and combinations BAP, Kn IAA for multiple shoot formation via organogenesis. 100% induction was observed when the supplemented with IAA. Earliest within four weeks. The media 1.5 mg/l BAP + 0.5 0.2 found to be best regards number required initiation regeneration, no. regeneration frequency in case both varieties. Shoot elongation obtained same medium. elongated shoots rooted half strength supplanted IBA. vitro plantlets successfully established soil 97% survival rate. Subsequently, transferred large earthen pot acclimatization finally, open field further growth development. Plant Tissue Cult. & Biotech. 32(1): 43-51, 2022 (June)
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ژورنال
عنوان ژورنال: Plant Tissue Culture and Biotechnology
سال: 2022
ISSN: ['1818-8745', '1817-3721']
DOI: https://doi.org/10.3329/ptcb.v32i1.60471